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TotalSeq™-B0842 anti-mouse Ly-49A Antibody
产品名称:
TotalSeq™-B0842 anti-mouse Ly-49A Antibody
产品类别:
抗体
产品编号:
116815
产品应用:
116815
[价格]
规格 价格 库存
10µg ¥ 5166 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Con A activated splenic T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation
The antibody was purified by chromatography and conjugated with TotalSeq?-B oligomer under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)

1. Takei F. 1983. J. Immunol. 130:2794.
2. Chan P, et al. 1989. J. Immunol. 142:1727.

RRID
AB_2888910 (BioLegend Cat. No. 116815)

Antigen Details

Structure
C-type lectin, Ly-49, ITIM, 44 kD
Distribution

NK and NK-T cells

Function
Inhibitory NK cell receptor
Ligand/Receptor
MHC Class I Molecules
Cell Type
NK cells, NKT cells
Biology Area
Immunology
Antigen References

1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236.
2. Takei FJ, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.

Gene ID
16627 View all products for this Gene ID
UniProt
View information about Ly-49A on UniProt.org

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