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Alexa Fluor® 488 anti-mouse IFN-γ Antibo
产品名称：
Alexa Fluor® 488 anti-mouse IFN-γ Antibo
产品类别：
抗体
产品编号：
505815
产品应用：
505815
[价格]
规格 价格 库存
25µg ￥ 1568 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.
ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The LEAF? purified antibody is suggested for ELISPOT capture (Cat. No. 505812).
Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations.
Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-?. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.
Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut)
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
  3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut)
  4. Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
  6. Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
  7. Ferrick D, et al. 1995. Nature 373:255. (FC)
  8. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  9. Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut)
  10. DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut)
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed
  14. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  15. Montfort M, et al.2004. J. Immunol. 173:4084. PubMed
  16. Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
  17. Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed
  18. Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed
  19. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  20. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
  21. Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed
  22. Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
  23. Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)
Product Citations
  1. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  2. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  3. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  4. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  5. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  6. Lucas B, et al. 2020. Nat Commun. 11:2198. PubMed
  7. Li A, et al. 2020. J Virol. 94:00:00. PubMed
  8. Moulin M, et al. 2017. Cell Mol Life Sci. 10.1007/s00018-017-2583-0. PubMed
  9. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
  10. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  11. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  12. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  13. Montfort M, et al. 2004. J Immunol. 173:4084. PubMed
  14. Śledzińska A, et al. 2020. Immunity. 52(1):151-166.e6.. PubMed
  15. Meyer Zu Horste G et al. 2018. Immunity. 48(3):556-569 . PubMed
  16. Dai K, et al. 2015. J Leukoc Biol. 98: 1071 - 1080. PubMed
  17. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  18. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  19. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  20. Lee J, et al. 2007. Nat Immunol. 8:181. PubMed
  21. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  22. Nanishi E, et al. 2022. Commun Biol. 5:790. PubMed
  23. Baja?a S, et al. 2022. iScience. 25:103732. PubMed
  24. T?htinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  25. Mykk?nen O, et al. 2014. PLoS One. 9:114790. PubMed
  26. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  27. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  28. Han H, et al. 2020. Cancer Cell. 36(5):483-497.e15.. PubMed
RRID
AB_493313 (BioLegend Cat. No. 505815) AB_493312 (BioLegend Cat. No. 505813)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

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