Purified anti-mouse CD64 (FcγRI) Antibody
- 产品名称：
- Purified anti-mouse CD64 (FcγRI) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 139301
- 产品应用：
- 139301
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- BALB/c mouse FcγRI-human IgG Fc fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
- Application References
(PubMed link indicates BioLegend citation) -
- Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
- Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
- Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
- Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Melo GD, et al. 2017. Sci Rep. 6.1625. PubMed
- Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
- Arimura K, et al. 2017. Mucosal Immunol. 1.08125. PubMed
- Orecchioni M, et al. 2022. Methods Mol Biol. 2419:779. PubMed
- Rissiek B, et al. 2017. Sci Rep. . 10.1038/s41598-017-16613-w. PubMed
- Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
- Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
- Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
- Feuerstein R, et al. 2020. Elife. 9:00. PubMed
- Barvalia M, et al. 2022. Methods Mol Biol. 2508:147. PubMed
- Ozeri E, et al. 2012. J Immunol. 189:146. PubMed
- Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
- Blriot C, et al. 2021. Immunity. :. PubMed
- Janela B, et al. 2019. Immunity. 50:1069. PubMed
- Gubin MM, et al. 2018. Cell. 175:1014. PubMed
- Galbas T, et al. 2017. J Immunol. 198(2):852-861. PubMed
- Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
- Qiu Z, et al. 2022. Cancer Discov. 12:502. PubMed
- Gong P, et al. 2022. Cell Death Discov. 8:466. PubMed
- Diao J, et al. 2018. J Immunol. 201:1306. PubMed
- Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
- Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
- K?stele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
- RRID
- AB_10612757 (BioLegend Cat. No. 139301) AB_10613107 (BioLegend Cat. No. 139302)
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
-
Monocytes, macrophages, mast cells, dendritic cells
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG
- Cell Type
- Dendritic cells, Macrophages, Mast cells, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14129 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
