PerCP/Cyanine5.5 anti-mouse CD64 (FcγRI) Ant
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD64 (FcγRI) Ant
- 产品类别：
- 抗体
- 产品编号：
- 139307
- 产品应用：
- 139307
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- BALB/c mouse FcγRI-human IgG Fc fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
- Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
- Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
- Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
- van Wijck Y, et al. 2018. J Immunol. 200:1570. PubMed
- Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
- Pisu D, et al. 2020. STAR Protoc. 1:100123. PubMed
- De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed
- Dodagatta-Marri E, et al. 2021. Cell Rep. 36:109309. PubMed
- Marinaik CB, et al. 2020. Cell Rep Med. 1:100095. PubMed
- Baasch S, et al. 2021. Cell. . PubMed
- Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
- Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed
- Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
- Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
- Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
- Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
- Pisu D, et al. 2020. Cell Rep. 30:335. PubMed
- Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
- Lee W, et al. 2021. J Virol. 95:e0053021. PubMed
- Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
- Shibata T, et al. 2020. J Clin Invest. 130:3021. PubMed
- Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed
- Sung PS, et al. 2022. JCI Insight. :. PubMed
- Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed
- Hakim R, et al. 2021. J Neurosci. 41:8441. PubMed
- Feuerstein R, et al. 2015. J Immunol. 194:2735. PubMed
- Kolter J, et al. 2016. J Immunol. 196: 2733 - 2741. PubMed
- Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed
- Subramanian S, et al. 2022. Nat Immunol. 23:458. PubMed
- Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
- Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed
- Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed
- RRID
- AB_2561962 (BioLegend Cat. No. 139307) AB_2561963 (BioLegend Cat. No. 139308)
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
-
Monocytes, macrophages, mast cells, dendritic cells
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG
- Cell Type
- Dendritic cells, Macrophages, Mast cells, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14129 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
