PE/Dazzle™ 594 anti-mouse CD64 (FcγRI)
- 产品名称：
- PE/Dazzle™ 594 anti-mouse CD64 (FcγRI)
- 产品类别：
- 抗体
- 产品编号：
- 139319
- 产品应用：
- 139319
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- BALB/c mouse FcγRI-human IgG Fc fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
- Application References
(PubMed link indicates BioLegend citation) -
- Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
- Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
- Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
- Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
- Sepe JJ, et al. 2022. JACC Basic Transl Sci. 7:915. PubMed
- Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
- Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
- Marangoni F, et al. 2021. Cell. . PubMed
- Lu S, et al. 2020. JCI Insight. 5:00. PubMed
- Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
- Forbester JL, et al. 2020. J Virol. 94:. PubMed
- Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
- Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
- White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
- Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
- RRID
- AB_2566558 (BioLegend Cat. No. 139319) AB_2566559 (BioLegend Cat. No. 139320)
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
-
Monocytes, macrophages, mast cells, dendritic cells
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG
- Cell Type
- Dendritic cells, Macrophages, Mast cells, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14129 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
