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首页 > 抗体蛋白 > 抗体
PE/Cyanine7 anti-mouse CD64 (FcγRI) Antibody
产品名称：
PE/Cyanine7 anti-mouse CD64 (FcγRI) Antibody
产品类别：
抗体
产品编号：
139313
产品应用：
139313
[价格]
规格 价格 库存
25µg ￥ 2464 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  6. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  3. Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed
  4. Larson-Casey JL, et al. 2021. FASEB J. 35:e21675. PubMed
  5. B?ttcher JP, et al. 2018. Cell. 172:1022. PubMed
  6. Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed
  7. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  8. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  9. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  10. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  11. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  12. Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed
  13. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  14. Affo S, et al. 2021. Cancer Cell. 39(6):866-882.e11. PubMed
  15. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  16. Hole CR, et al. 2019. Nat Commun. 10:2955. PubMed
  17. Freemerman AJ, et al. 2019. J Immunol. 202:1265. PubMed
  18. Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed
  19. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  20. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  21. Yadav P, et al. 2021. J Immunol. 206:2888. PubMed
  22. Liu CY, et al. 2020. Cell Rep. 33:108275. PubMed
  23. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  24. Wu Y, et al. 2021. PLoS Negl Trop Dis. 15:e0010050. PubMed
  25. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  26. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  27. Casulli J, et al. 2019. Nat Commun. 10:2121. PubMed
  28. Zheng J, et al. 2020. Nature. . PubMed
  29. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
  30. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  31. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  32. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  33. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  34. Larson-Casey JL, et al. 2021. J Biol Chem. :100810. PubMed
  35. Giampazolias E, et al. 2021. Cell. . PubMed
  36. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  37. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  38. Chen F, et al. 2022. Cell Rep. 38:110215. PubMed
  39. Xu J, et al. 2020. Immunity. 53(2):371-383.e5. PubMed
  40. Birkl D, et al. 2019. FASEB J. 33:13632. PubMed
  41. Carlson PM, et al. 2021. J Immunol. 207:720. PubMed
  42. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  43. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  44. Choi J, et al. 2020. Cell Stem Cell. 27:366. PubMed
  45. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  46. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  47. Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed
  48. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  49. Larson–Casey JL, et al. 2019. J Clin Invest. 129:4962. PubMed
  50. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  51. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_2563903 (BioLegend Cat. No. 139313) AB_2563904 (BioLegend Cat. No. 139314)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

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