Brilliant Violet 421™ anti-mouse CD64 (Fc&ga
- 产品名称：
- Brilliant Violet 421™ anti-mouse CD64 (Fc&ga
- 产品类别：
- 抗体
- 产品编号：
- 139309
- 产品应用：
- 139309
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- BALB/c mouse FcγRI-human IgG Fc fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.75 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
- Application References
(PubMed link indicates BioLegend citation) -
- Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
- Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
- Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
- Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Liu J, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.139. PubMed
- Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
- Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
- Chikina AS, et al. 2020. Cell. 183:411. PubMed
- Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed
- Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
- Liu J, et al. 2020. Am J Pathol. 190:1298. PubMed
- Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed
- Kovács B, et al. 2020. Front Immunol. 2.508333333. PubMed
- Kedage V, et al. 2022. MAbs. 14:2040083. PubMed
- Ku MW, et al. 2020. Cell Host Microbe. 29(2):236-249.e6. PubMed
- Zhang S, et al. 2019. Cell Metab. 29:443. PubMed
- Schippers A, et al. 2015. Mucosal Immunol. 101038/mi201582. PubMed
- Matheis F, et al. 2020. Cell. 180(1):64-78.e16.. PubMed
- Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
- Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
- Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
- Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
- Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
- Branchett WJ, et al. 2020. Wellcome Open Research. 0.278472222. PubMed
- Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
- Draheim M, et al. 2017. EMBO Mol Med. 1.489583333. PubMed
- Riding AM, et al. 2022. iScience. 25:104660. PubMed
- Gubin MM, et al. 2018. Cell. 175:1014. PubMed
- Martinez-Turtos A, et al. 2022. Oncoimmunology. 11:2116844. PubMed
- Wang Y, et al. 2020. eLife. 9:e57438.. PubMed
- Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed
- Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
- Liu J, et al. 2018. Am J Pathol. 188:2786. PubMed
- Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
- Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
- E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
- Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
- Sharma M, et al. 2019. Immunometabolism. 1. PubMed
- Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
- Barnette DN, et al. 2018. JCI Insight. 3. PubMed
- Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
- Lai NY, et al. 2020. Cell. 180:33:00. PubMed
- Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
- Jones GS, et al. 2020. mSphere. 5:. PubMed
- Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
- Rivera CA, et al. 2022. Immunity. 55:129. PubMed
- Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
- Fearon AE, et al. 2021. iScience. 24:103143. PubMed
- RRID
- AB_2562694 (BioLegend Cat. No. 139309)
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
-
Monocytes, macrophages, mast cells, dendritic cells
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG
- Cell Type
- Dendritic cells, Macrophages, Mast cells, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14129 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
