Purified anti-human CD33 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1428	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human myeloid leukaemia cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified
ICC, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting³, induction of cytokine production³, and immunofluorescence⁴. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)

Product Citations

Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
Wen J, et al. 2020. J Pathol Clin Res. 0.36875. PubMed
Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
Zhang Y, et al. 2020. Int J Mol Sci. 21:00. PubMed
Zhang Y, et al. 2022. Exp Hematol Oncol. 11:15. PubMed
Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
Henrick BM, et al. 2021. Cell. . PubMed
Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
Hou Z, et al. 2020. Oncol Lett. 19:3982. PubMed
Crawford LB, et al. 2021. J Virol. 95:. PubMed
Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed

RRID

AB_314346 (BioLegend Cat. No. 303402)

Antigen Details

Structure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
Function: Adhesion and lectin activity
Ligand/Receptor: Sugar chains containing sialic acid
Cell Type: B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.
Gene ID: 945 View all products for this Gene ID
UniProt: View information about CD33 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们