PerCP/Cyanine5.5 anti-human CD33 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2464	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human myeloid leukaemia cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting³, induction of cytokine production³, and immunofluorescence⁴. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)

Product Citations

Capietto A, et al. 2013. J Exp Med. 210:2257. PubMed
Le Q, et al. 2021. Clin Cancer Res. Online ahead of prin. PubMed
Kuen J, et al. 2017. PLoS One. 10.1371/journal.pone.0182039. PubMed
Wu L, et al. 2022. Theranostics. 12:842. PubMed
Yankova E, et al. 2021. Nature. 593:597. PubMed
Kraus H, et al. 2014. J Immunol. 192:1044. PubMed
Norton S, et al. 2016. Clin Transl Immunology. 5: e76. PubMed
Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed
Spector A 2014. J Infect Dis. 209:441. PubMed

RRID

AB_2074242 (BioLegend Cat. No. 303413) AB_2074241 (BioLegend Cat. No. 303414)

Antigen Details

Structure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
Function: Adhesion and lectin activity
Ligand/Receptor: Sugar chains containing sialic acid
Cell Type: B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.
Gene ID: 945 View all products for this Gene ID
UniProt: View information about CD33 on UniProt.org

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