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PE anti-human CD33 Antibody
产品名称：
PE anti-human CD33 Antibody
产品类别：
抗体
产品编号：
303403
产品应用：
303403
[价格]
规格 价格 库存
25tests ￥ 1498 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human myeloid leukaemia cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
  3. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
  4. Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
Product Citations
  1. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  2. Galán-Díez M, et al. 2022. Cancer Discov. . PubMed
  3. Tcheng M, et al. 2021. Blood. 137:3518. PubMed
  4. Zhu ZY, et al. 2022. Front Immunol. 12:781087. PubMed
  5. Subramaniam A, et al. 2020. Blood. 136:2151. PubMed
  6. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  7. Schmiderer L, et al. 2020. Proc Natl Acad Sci U S A. 117:21267. PubMed
  8. Eriksson E, et al. 2019. J Immunol. 202:787. PubMed
  9. Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
  10. Szentkereszty M, et al. 2020. Pathol Oncol Res. 26:1117. PubMed
  11. Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed
  12. Salik B, et al. 2020. Cancer Cell. 38(2):263-278.e6. PubMed
  13. Blaszczak AM, et al. 2019. J Diabetes Res. 2019:8124563. PubMed
  14. Han AR, et al. 2021. Int J Stem Cells. 14:203. PubMed
  15. Lin G, et al. 2021. Mol Med Rep. 23:. PubMed
  16. Sade-Feldman M, et al. 2016. Clin Cancer Res. 22(23):5661-5672. PubMed
  17. Danzer H, et al. 2020. Elife. 9:00. PubMed
  18. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  19. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  20. Chou DB, et al. 2020. Nat Biomed Eng. 0.440277778. PubMed
RRID
AB_314347 (BioLegend Cat. No. 303403) AB_314348 (BioLegend Cat. No. 303404)

Antigen Details

Structure
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution

Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells

Function
Adhesion and lectin activity
Ligand/Receptor
Sugar chains containing sialic acid
Cell Type
B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org

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