PE/Cyanine7 anti-human CD45RO Antibody
- 产品名称：
- PE/Cyanine7 anti-human CD45RO Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304230
- 产品应用：
- 304230
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 4858 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cynomolgus, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- IL-2 dependent T cell line, CA1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and na?ve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
- Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
- Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
- Yamada M, et al. 1996. Stroke 27:1155. (IHC)
- Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
- Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
- Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
- Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
- Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Gordeychuk IV, et al. 2019. Acta Naturae. 10:63. PubMed
- Kieffer T, et al. 2017. J Reprod Immunol. 10.1016/j.jri.2016.11.004. PubMed
- Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
- Traitanon O, et al. 2015. PLoS One. 10:129658. PubMed
- Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
- Cao W, et al. 2017. Immunity. 47:1182. PubMed
- Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
- Zhou WJ, et al. 2021. Theranostics. 11:5330. PubMed
- Gurusamy D, et al. 2020. Cancer Cell. 37(6):818-833.e9. PubMed
- Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
- Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
- Jiang W, et al. 2014. J Virol. 88:5369. PubMed
- Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
- Leeansyah E, et al. 2014. Nat Commun. 5:3143. PubMed
- Huang L, et al. 2017. Immun Ageing. 10.1186/s12979-017-0096-1. PubMed
- Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
- Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
- Delmas A, et al. 2016. PLoS One. 11: 0163305. PubMed
- Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
- Mann EH, et al. 2019. J Immunol. 202:684. PubMed
- RRID
- AB_11203903 (BioLegend Cat. No. 304229) AB_11203900 (BioLegend Cat. No. 304230)
- Structure
- Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
- Distribution
-
Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- CD22
- Cell Type
- B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RO on UniProt.org
