Ultra-LEAF™ Purified anti-human CD13 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 4466	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Baboon, Chimpanzee, Cotton-topped Tamarin

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: inhibition of tumor-cell invasion and blocking of aminopeptidase activities^2,3, and immunohistochemical staining of acetone-fixed frozen tissue sections⁵. WM15 does not?recognize formalin-fixed or paraffin-embedded tissue sections⁵. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301708). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 301723 and 301724) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Saiki I, et al. 1993. Int J Cancer. 54:137. (Block)
Rosenzwajg M, et al. 2000. Blood 95:453. (Block)
Kawase M, et al. 2008. J Virol. 83:712. (Block) PubMed
Di Matteo P, et al. 2011. J. Histochem. Cytochem. 59:47. (IHC)

RRID

AB_2728236 (BioLegend Cat. No. 301723) AB_2728237 (BioLegend Cat. No. 301724)

Antigen Details

Structure: Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD
Distribution: Granulocytes, monocytes, myeloid progenitors, endothelial and epithelial cells, granular lymphocyte subset
Function: Zinc-binding metalloproteinase, antigen processing, cleaves MIP-1 chemokine
Ligand/Receptor: Coronavirus receptor
Cell Type: Endothelial cells, Epithelial cells, Granulocytes, Hematopoietic stem and progenitors, Lymphocytes, Mesenchymal Stem Cells, Monocytes, Neutrophils
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Shipp M, et al. 1993. Blood 82:1052.
2. Larsen S, et al. 1996. J. Exp. Med. 184:183.
Gene ID: 290 View all products for this Gene ID
UniProt: View information about CD13 on UniProt.org

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