Ultra-LEAF™ Purified anti-mouse CD273 (B7-DC-上海雅吉生物科技有限公司

规格	价格	库存
5mg	￥ 7546	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse B7-DC transfected cell line

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting^1,6, blocking^2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections³. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107221-107226).

Application References (PubMed link indicates BioLegend citation)

Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC, IP, WB)
Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block)
Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC)
Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC, Block)
Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block)
Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Murakami R, et al. 2013. PLoS One. 8:73270. PubMed

RRID

AB_2813956 (BioLegend Cat. No. 107221) AB_2813957 (BioLegend Cat. No. 107222) AB_2813958 (BioLegend Cat. No. 107223) AB_2813959 (BioLegend Cat. No. 107224) AB_2813960 (BioLegend Cat. No. 107225) AB_2813961 (BioLegend Cat. No. 107226)

Antigen Details

Structure: B7 Immunoglobulin superfamily, 42 kD
Distribution: Dendritic cells, liver, few transformed cell lines, subset of macrophages; not expressed on lymphoid tissues
Function: Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor: PD-1, uncharacterized receptor
Cell Type: Dendritic cells, Macrophages
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Shin T, et al. 2003. J. Exp. Med. 198:31.
2. Liu X, et al. 2003. J. Exp. Med. 197:1721.
3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
Gene ID: 58205 View all products for this Gene ID
UniProt: View information about CD273 on UniProt.org

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