PerCP/Cyanine5.5 anti-rat CD4 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1736	1

Product Details

Verified Reactivity

Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Rat thymocyte membrane glycoproteins

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^1,2, inhibition of IL-2 production³, inhibition of MBP-induced T cell activation in EAE transfer model³.

Application References (PubMed link indicates BioLegend citation)

Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
Mannie MD, et al. 1993. J. Immunol. 151:7293.
Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed

Product Citations

Giri T, et al. 2022. Heliyon. 8:e09407. PubMed

RRID

AB_2814097 (BioLegend Cat. No. 201519) AB_2814098 (BioLegend Cat. No. 201520)

Antigen Details

Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
Function: T cell co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Macrophages, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.
Gene ID: 24932 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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