APC/Cyanine7 anti-rat CD4 Antibody
- 产品名称：
- APC/Cyanine7 anti-rat CD4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 201517
- 产品应用：
- 201517
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat thymocyte membrane glycoproteins
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.
- Additional Product Notes
- BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
- Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
- Mannie MD, et al. 1993. J. Immunol. 151:7293.
- Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
- Product Citations
-
- Xie L, et al. 2014. J Immunol. 192:6009. PubMed
- Fehrenbach DJ, et al. 2020. Exp Physiol. 105:864. PubMed
- Abais-Battad JM, et al. 2021. Acta Physiol (Oxf). 232:e13662. PubMed
- Pai AV, et al. 2018. Physiology (Bethesda). 33:254. PubMed
- Alsheikh AJ, et al. 2020. Am J Physiol Renal Physiol. F982:318. PubMed
- Yanai H, et al. 2022. Elife. 11:. PubMed
- Li B, et al. 2020. Cell Mol Immunol. 1.386111111. PubMed
- Dasinger JH, et al. 2021. Pregnancy Hypertens. 24:126. PubMed
- Almolda B, et al. 2011. PLoS One. 6:e27473. PubMed
- Alsheikh AJ, et al. 2019. Am J Physiol Regul Integr Comp Physiol. 317:R182. PubMed
- Ruppert KA, et al. 2018. Sci Rep. 8:480. PubMed
- RRID
- AB_1186088 (BioLegend Cat. No. 201517) AB_1186084 (BioLegend Cat. No. 201518)
