APC anti-rat CD4 Antibody
- 产品名称：
- APC anti-rat CD4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 201509
- 产品应用：
- 201509
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat thymocyte membrane glycoproteins
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.
- Application References
(PubMed link indicates BioLegend citation) -
- Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
- Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
- Mannie MD, et al. 1993. J. Immunol. 151:7293.
- Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
- Product Citations
-
- Wang Y, et al. 2021. Front Cell Dev Biol. 9:702890. PubMed
- Zhang HC, et al. 2018. Int J Mol Med. 41:13. PubMed
- Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
- Treacy O, et al. 2012. PLoS One. 7:e42662. PubMed
- Ni X, et al. 2019. Mol Med Rep. 19:3743. PubMed
- RRID
- AB_313935 (BioLegend Cat. No. 201509)
- Structure
- Ig superfamily, 55 kD
- Distribution
-
Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
- Function
- T cell co-receptor, T cell activation
- Ligand/Receptor
- MHC class II molecule
- Cell Type
- Macrophages, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.
- Gene ID
- 24932 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
