Alexa Fluor® 488 anti-rat CD4 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1414	1

Product Details

Verified Reactivity

Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Rat thymocyte membrane glycoproteins

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Blue Laser (488 nm)

Application Notes

The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^1,2, inhibition of IL-2 production³, inhibition of MBP-induced T cell activation in EAE transfer model³.

Application References (PubMed link indicates BioLegend citation)

Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
Mannie MD, et al. 1993. J. Immunol. 151:7293.
Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed

Product Citations

Nuccitelli R, et al. 2015. PLoS One. 10: 0134364. PubMed
Xiao L, et al. 2014. J Endod. 41:479. PubMed
Qiu T, et al. 2022. Cancers (Basel). 14:. PubMed

RRID

AB_572038 (BioLegend Cat. No. 201511)

Antigen Details

Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
Function: T cell co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Macrophages, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.
Gene ID: 24933 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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