Alexa Fluor® 647 anti-rat CD43 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3710	1

Product Details

Verified Reactivity

Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Rat thymocyte membrane glycoproteins

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections¹ and paraffin-embedded sections⁴.

Application References (PubMed link indicates BioLegend citation)

Barclay AN. 1981. Immunology 42:593.
Brown WRA, et al. 1981. Nature 289:456.
Forbes RC, et al. 1983. Transplantation 36:681.
Jung S, et al. 1994. Immunology 83:545.

Product Citations

Abbondanzo S, Chang S 2014. PLoS One. 9:105256. PubMed
Kiefer J, et al. 2021. Front Immunol. 12:641224. PubMed

RRID

AB_2194199 (BioLegend Cat. No. 202810)

Antigen Details

Structure

Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130kD

Distribution

Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.

Function

Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors

Ligand/Receptor

CD54, Siglec-1

Cell Type

T cells, NK cells, Granulocytes, Monocytes, Thymocytes, Platelets, Plasma cells, B cells, Hematopoietic stem and progenitors

Biology Area

Immunology

Molecular Family

CD Molecules

Antigen References

Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
Killeen A, et al. 1987. EMBO J. 6(13):4029.
Thurman EC, em>et al. 1998. Intl. Immunol. 10:691.
Onami TM, et al. 2002. J. Immunol. 168:6022.
Tong J, et al. 2004. J. Exp. Med. 199:1277.
Jones AT, et al. 1994. J. Immunol. 153:3426.

Gene ID