Direct-Blot™ HRP anti-Hsp70 Antibody
- 产品名称：
- Direct-Blot™ HRP anti-Hsp70 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 648005
- 产品应用：
- 648005
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Hsp70 protein from HeLa cells.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
- RRID
- AB_2566707 (BioLegend Cat. No. 648005)
- Structure
- Hsp proteins are the most conserved proteins present in both prokaryotes and eukaryotes. Human HSp70 contains multiple isoforms. Hsp70s consisit of three structural domains, a 44Kd amino-terminal ATPase domain (NBD), followed by an 18 Kd substrate
- Distribution
-
Ubiquitous, cytoplasmic and nuclear
- Function
- Molecular chaperrones assisting in the folding of proteins and preventing protein aggregation
- Biology Area
- Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
- Antigen References
-
1. Sharma D, et al. 2009. Protein Peptide Lett. 16:571.
2. Johnson JD, et al. 2006. J. Leuk. Biol. 79:425.
3. Bukau B, et al. 1998. Cell 92:351.
- Gene ID
- 3308 View all products for this Gene ID
- UniProt
- View information about Hsp70 on UniProt.org
