Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin
- 产品类别：
- 抗体
- 产品编号：
- 138107
- 产品应用：
- 138107
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- VE-cadherin protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for relevant formats) include: Immunoprecipitation1, Western Blot1, functional assay2, 3 by inhibiting vascular endothelial cell-cell interactions and immunocytochemistry staining of cultured cell lines4.
- Application References
(PubMed link indicates BioLegend citation) -
- Allport JR, et al. 2002. J. Leukoc. Biol. 71:821. (IP WB)
- Hirashima M, et al. 2009. Blood 93:1253. (Block)
- Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. (Block)
- Matsumura K, et al. 2003. Blood 101:1367. (IF)
- Hirashima M, et al. 2009. Blood 101:2261. (FC)
- Product Citations
-
- Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
- RRID
- AB_10643578 (BioLegend Cat. No. 138107) AB_10643579 (BioLegend Cat. No. 138108)
- Structure
- A 120 kD member of type II Cadherin family
- Distribution
- Endothelial cells
- Function
- Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion.
- Cell Type
- Endothelial cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
2. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.
- Gene ID
- 12562 View all products for this Gene ID
- UniProt
- View information about CD144 on UniProt.org
