Purified anti-Cyclin B1 Antibody
- 产品名称：
- Purified anti-Cyclin B1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 647901
- 产品应用：
- 647901
- Verified Reactivity
- Human
- Reported Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Hamster Cyclin B1
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2.5 - 5.0 ?g antibody per 5 mL?antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications include: immunohistochemistry2 of formalin-fixed paraffin-embedded sections and immunofluorescence1 of methanol fixed cells grown on glass coverslips.
- Application References
(PubMed link indicates BioLegend citation) -
- Geley S, et al. 2001. J. Cell Biol. 153:137-148 PubMed.
- Brandeis M, et al. 1998. P. Natl. Acad. Sci. USA 95:4344-4349. PubMed
- Matsumura F, et al. 2011. J. Biol Chem. PubMed
- Product Citations
-
- Brazzo JA, et al. 2021. J Cell Sci. 134:. PubMed
- Matsumura F, et al. 2011. J Biol Chem. 286:10825. PubMed
- Neumann-Staubitz P, et al. 2022. Front Chem. 10:905794. PubMed
- Iii JAB, et al. 2021. J Cell Sci. . PubMed
- RRID
- AB_2072274 (BioLegend Cat. No. 647901) AB_2244210 (BioLegend Cat. No. 647902)
- Structure
- Belongs to the cyclin family. Cyclin AB subfamily.
- Distribution
-
Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.
- Interaction
- CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4
- Bioactivity
- Essential for the control of the cell cycle at the G2/M (mitosis) transition.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
-
1. Morgan DO, et al.1995. Nature 374:131
- Gene ID
- 891 View all products for this Gene ID
- UniProt
- View information about Cyclin B1 on UniProt.org
