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PE anti-Cyclin B1 Antibody
产品名称:
PE anti-Cyclin B1 Antibody
产品类别:
抗体
产品编号:
647903
产品应用:
647903
[价格]
规格 价格 库存
25tests ¥ 2142 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Hamster Cyclin B1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunohistochemistry2 of formalin-fixed paraffin-embedded sections and immunofluorescence1 of methanol fixed cells grown on glass coverslips.

Application References

(PubMed link indicates BioLegend citation)
  1. Geley S, et al. 2001. J. Cell Biol. 153:137-148 PubMed.
  2. Brandeis M, et al. 1998. P. Natl. Acad. Sci. USA 95:4344-4349. PubMed
  3. Matsumura F, et al. 2011. J. Biol Chem. PubMed
Product Citations
  1. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
RRID
AB_2616693 (BioLegend Cat. No. 647903) AB_2616701 (BioLegend Cat. No. 647904)

Antigen Details

Structure
Belongs to the cyclin family. Cyclin AB subfamily.
Distribution

Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.

Interaction
CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4
Bioactivity
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Morgan DO, et al.1995. Nature 374:131

Gene ID
891 View all products for this Gene ID
UniProt
View information about Cyclin B1 on UniProt.org

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