Alexa Fluor® 647 anti-Cyclin B1 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-Cyclin B1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 647905
- 产品应用：
- 647905
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2058 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Hamster Cyclin B1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications include: immunohistochemistry2 of formalin-fixed paraffin-embedded sections and immunofluorescence1 of methanol fixed cells grown on glass coverslips.
- Application References
(PubMed link indicates BioLegend citation) -
- Geley S, et al. 2001. J. Cell Biol. 153:137-148 PubMed.
- Brandeis M, et al. 1998. P. Natl. Acad. Sci. USA 95:4344-4349. PubMed
- Matsumura F, et al. 2011. J. Biol Chem. PubMed
- Product Citations
-
- Amar-Schwartz A, et al. 2022. Elife. 11:. PubMed
- RRID
- AB_2632637 (BioLegend Cat. No. 647905) AB_2632638 (BioLegend Cat. No. 647906)
- Structure
- Belongs to the cyclin family. Cyclin AB subfamily.
- Distribution
-
Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.
- Interaction
- CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4
- Bioactivity
- Essential for the control of the cell cycle at the G2/M (mitosis) transition.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
-
1. Morgan DO, et al.1995. Nature 374:131
- Gene ID
- 891 View all products for this Gene ID
- UniProt
- View information about Cyclin B1 on UniProt.org
