Alexa Fluor® 647 anti-human CD243 (MDR-1) Anti
- 产品名称：
- Alexa Fluor® 647 anti-human CD243 (MDR-1) Anti
- 产品类别：
- 抗体
- 产品编号：
- 348613
- 产品应用：
- 348613
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NIH 3T3 cells transfected with human MDR-1 cDNA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC, IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: blocking the efflux of fluorescent dyes1,2, immunoprecipitation1, and immunohistochemical staining of tissue sections of squamous cell carcinoma3.
- Application References
(PubMed link indicates BioLegend citation) -
- Mechetner EB and Roninson IB. 1992.?Proc. Natl. Acad. Sci. USA?13:5824. (Block, IP)
- Chaudhary PM,?et al.?1992.?Blood?80:2735. (Block)
- Kelley DJ,?et al.?1993.?Arch. Otolaryngol. Head Neck Surg.?119:411. (IHC-P)
- Goda K,?et al.?2007.?J. Pharmacol. Exp. Ther.?320:81. (Block)
- RRID
- AB_2734356 (BioLegend Cat. No. 348613) AB_2734357 (BioLegend Cat. No. 348614)
- Structure
- Member of ABC transporters family, MW ~170 kD. Consists of two homologous halves, each comprising two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs).
- Distribution
-
Hematopoietic stem cells, T and B cells, natural killer (NK) cells, and many multidrug resistant neoplastic cells.
- Function
- Transport various molecules across cellular membranes. Involved in multidrug resistance.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Dulucq S,?et al.?2008.?Blood?112:2024.?
2. Gottesman MM,?et al.?2009.?Nat. Biotechnol.?27:546.?
3. Andreadis C,?et al.?2007.?Blood?109:3409.
- Gene ID
- 5243 View all products for this Gene ID
- UniProt
- View information about CD243 on UniProt.org
