Pacific Blue™ anti-human CD5 Antibody
- 产品名称：
- Pacific Blue™ anti-human CD5 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300623
- 产品应用：
- 300623
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Capuchin Monkey, Common Marmoset, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting2 and immunohistochemical staining of acetone-fixed frozen sections2,5.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
- Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC)
- Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437.
- Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed
- Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
- RRID
- AB_2075458 (BioLegend Cat. No. 300623) AB_2075457 (BioLegend Cat. No. 300624)
- Structure
- Scavenger receptor superfamily, 67 kD
- Distribution
-
T cells, thymocytes, B cell subset, B cell CLL, peripheral blood dendritic cells
- Function
- Modulates TCR, BCR signaling, thymocyte maturation, T-B cell interaction
- Ligand/Receptor
- CD72
- Cell Type
- B cells, Dendritic cells, Leukemia, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kipps T. 1988. Adv. Immunol. 47:117.
2. Resnick D, et al. 1993. Trends Biochem. Sci. 19:5.
3. Wood GS, et al. 1992. Am. J. Pathol. 14:789.
- Gene ID
- 921 View all products for this Gene ID
- UniProt
- View information about CD5 on UniProt.org
