Spark Violet™ 538 anti-human CD3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1596	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark Violet? 538 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet? 538 has a maximum excitation of 396 nm and a maximum emission of 538 nm.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections¹¹, immunoprecipitation¹, activation of T cells^2,3,5, Western blotting⁹, and spatial biology (IBEX)^16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
Li J, et al. 2015. Cancer Res. 75:508. PubMed
Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

RRID

AB_2890733 (BioLegend Cat. No. 300483) AB_2890733 (BioLegend Cat. No. 300484)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

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