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Purified anti-human CD3 Antibody
产品名称：
Purified anti-human CD3 Antibody
产品类别：
抗体
产品编号：
300401
产品应用：
300401
[价格]
规格 价格 库存
25µg ￥ 336 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF?, IHC-F - Verified
IP, Activ, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. ?zcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed
  2. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  3. Mousset CM, et al. 2018. Oncoimmunology. 7:e1488565. PubMed
  4. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  5. Laurent A, et al. 2014. PLoS One. 9:103683. PubMed
  6. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  7. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  8. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  9. Nguyen LT, et al. 2019. Cancer Immunol Immunother. 68:773. PubMed
  10. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  11. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  12. González-Mancha N, et al. 2022. Front Immunol. 12:814570. PubMed
  13. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  14. Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed
  15. Kollis PM, et al. 2022. Front Immunol. 13:850226. PubMed
  16. Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed
  17. Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
  18. Bobardt M, et al. 2020. PLoS One. 15:e0227715. PubMed
  19. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  20. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  21. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  22. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  23. Wang C, et al. 2022. Exp Ther Med. 23:221. PubMed
  24. Coelho CH, et al. 2020. JCI Insight. 5:. PubMed
  25. Cribbs AP, et al. 2021. Front Immunol. 12:626255. PubMed
  26. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  27. Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
  28. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  29. Guo T, et al. 2018. J Immunol. 200:500. PubMed
  30. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  31. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  32. Michlmayr D, et al. 2020. Cell Reports. 31(4):107569. PubMed
  33. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  34. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  35. Hejazi M, et al. 2022. Front Immunol. 12:798087. PubMed
  36. Khanolkar A, et al. 2020. Immunohorizons. 4:153. PubMed
  37. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  38. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  39. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  40. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  41. Jakob M, et al. 2020. Cells. 10:. PubMed
  42. Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed
  43. Guerrero A, et al. 2012. J Immunol. 190:27. PubMed
  44. Wright CC, et al. 2017. Infect Immun. 85:e00131. PubMed
  45. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
  46. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  47. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  48. Hartmann FJ, et al. 2019. Cell Rep. 28:819. PubMed
  49. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  50. Schwabenland M, et al. 2021. Immunity. . PubMed
  51. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  52. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  53. Silva–Cardoso SC, et al. 2017. J Immunol. 199:253. PubMed
  54. Tang JS, et al. 2020. Food Funct. 11:5782. PubMed
  55. Thompson A, et al. 2004. J Immunol. 173:1671. PubMed
  56. Oda H, et al. 2019. Front Immunol. 10:479. PubMed
  57. Manes TL, et al. 2020. Free Radic Biol Med. 147:102. PubMed
  58. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  59. Han M, et al. 2022. Cell Mol Immunol. 19:805. PubMed
  60. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  61. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  62. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
  63. Martin E, et al. 2020. JCI Insight. :5. PubMed
  64. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  65. Yamaguchi K, et al. 2018. Cancer Sci. 109:3032. PubMed
  66. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  67. Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
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RRID
AB_314055 (BioLegend Cat. No. 300401) AB_314056 (BioLegend Cat. No. 300402)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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