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PE/Cyanine7 anti-human CD3 Antibody
产品名称：
PE/Cyanine7 anti-human CD3 Antibody
产品类别：
抗体
产品编号：
300419
产品应用：
300419
[价格]
规格 价格 库存
25tests ￥ 2002 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  2. Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
  3. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  4. Jiang L, et al. 2021. Int J Biol Sci. 17:4377. PubMed
  5. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  6. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  7. Vinjamur DS, et al. 2021. Nat Genet. 53:719. PubMed
  8. Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
  9. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  10. Goel G, et al. 2019. Sci Adv. 5:eaaw7756. PubMed
  11. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  12. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  13. Vijayakumar B, et al. 2022. Immunity. . PubMed
  14. Todorova D, et al. 2020. EBioMedicine. 62:103120. PubMed
  15. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  16. Ridley ML, et al. 2020. J Immunol. 204:2940. PubMed
  17. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  18. Seumois G, et al. 2016. J Immunol. 197: 655 - 664. PubMed
  19. Leone D, et al. 2016. PLoS One. 11: 0151674. PubMed
  20. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  21. Taraldsrud E, et al. 2017. Journal of Autoimmunity. 10.1016/j.jaut.2017.04.004. PubMed
  22. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  23. Ramirez NP, et al. 2022. Nat Commun. 13:1109. PubMed
  24. Usero L, et al. 2016. Diabetes. 65: 2356 - 2366. PubMed
  25. Meraviglia S, et al. 2017. Oncoimmunology. 6:e1347742. PubMed
  26. Zeng J, et al. 2020. Nat Med. 26:535. PubMed
  27. Cartellieri M, et al. 2014. PLoS One. 9:93745. PubMed
  28. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  29. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  30. Bonte S, et al. 2021. Oncoimmunology. 10:1954800. PubMed
  31. Alonso R, et al. 2022. Blood Adv. :. PubMed
  32. Vela M, et al. 2019. Sci Rep. 9:18792. PubMed
  33. Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed
  34. Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
  35. Pollack RA, et al. 2017. Cell Host Microbe. 1.218055556. PubMed
  36. Moore N, et al. 2019. Sci Rep. 9:15101. PubMed
  37. Comte D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9321 - 9326. PubMed
  38. Rosskopf S, et al. 2018. Oncotarget. 9:17608. PubMed
  39. Leone DA, et al. 2017. J Immunol. 199:531. PubMed
  40. Andreano E, et al. 2021. Nature. Online ahead of print. PubMed
  41. Cartellieri M, et al. 2016. Blood Cancer J. 6:e458. PubMed
  42. Iio K, et al. 2019. Sci Rep. 9:813. PubMed
  43. Román-Rodriguez FJ, et al. 2019. Cell Stem Cell. 25:607. PubMed
  44. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  45. Ishigaki K, et al. 2015. Sci Rep. 5: 12937. PubMed
  46. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  47. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  48. Gustafson CE, et al. 2020. JCI Insight. 5:. PubMed
  49. Gladkikh A, et al. 2017. Cancer Med. . 10.1002/cam4.1257. PubMed
  50. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
  51. Lo Presti E, et al. 2020. J Leukoc Biol. 108:749. PubMed
  52. Cruz-Lorenzo E, et al. 2022. Viruses. 14:. PubMed
  53. Andreano E, et al. 2021. Cell. 184(7):1821-1835.e16. PubMed
  54. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  55. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
  56. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
RRID
AB_439780 (BioLegend Cat. No. 300419) AB_439781 (BioLegend Cat. No. 300420)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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