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PE anti-human CD3 Antibody
产品名称：
PE anti-human CD3 Antibody
产品类别：
抗体
产品编号：
300407
产品应用：
300407
[价格]
规格 价格 库存
25tests ￥ 392 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Merah-Mourah F, et al. 2021. Curr Protoc. 1:e229. PubMed
  2. Groen R, et al. 2015. J Leukoc Biol. 98: 623 - 630. PubMed
  3. Miggitsch C, et al. 2019. EBioMedicine. 46:387. PubMed
  4. Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed
  5. Bessy T, et al. 2021. J Cell Biol. 220:. PubMed
  6. O’Connor K, et al. 2016. Genes Immun. 10.1038/gene.2016.27. PubMed
  7. Thakral D, et al. 2008. J Immunol. 180:7431. PubMed
  8. Mineo M, et al. 2020. Molecular Cell. 78(6):1207-1223.e8. PubMed
  9. Sun B, et al. 2019. Cancer Immunol Res. 1.550694444. PubMed
  10. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  11. Ramendra R, et al. 2021. Front Immunol. 12:694152. PubMed
  12. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  13. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  14. Nabet BY, et al. 2020. Cell. 183(2):363-376.e13. PubMed
  15. Hsiao CC, et al. 2022. STAR Protoc. 3:101422. PubMed
  16. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  17. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  18. Hirama T, et al. 2021. JCI Insight. 6:. PubMed
  19. Dong G, et al. 2020. Haematologica. . PubMed
  20. Wang X, et al. 2020. Exp Ther Med. 19:3698. PubMed
  21. Georg P, et al. 2022. Cell. 185:493. PubMed
  22. Zhao NQ, et al. 2020. PLoS One. 15:e0238347. PubMed
  23. Tomita K, et al. 2013. Neuroscience. 236:55. PubMed
  24. Singh KS, et al. 2021. Nature. 589:597. PubMed
  25. Yu W, et al. 2022. Front Cell Dev Biol. 10:928140. PubMed
  26. Lanz AL, et al. 2021. Cell Reports. 36(2):109375. PubMed
  27. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  28. Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
  29. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  30. Tiwarekar V, et al. 2018. J Virol. 92:17. PubMed
  31. Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed
  32. Deng W, et al. 2021. Emerg Microbes Infect. 10:1272. PubMed
RRID
AB_314061 (BioLegend Cat. No. 300407) AB_314062 (BioLegend Cat. No. 300408) AB_2564150 (BioLegend Cat. No. 300456) AB_2562047 (BioLegend Cat. No. 300441)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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