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Pacific Blue™ anti-human CD3 Antibody
产品名称：
Pacific Blue™ anti-human CD3 Antibody
产品类别：
抗体
产品编号：
300418
产品应用：
300418
[价格]
规格 价格 库存
25µg ￥ 1568 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
test size: lot-specific; ?g sizes: 0.5 mg/ml
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
For ?g sizes, the suggested use of this reagent for immunofluorescent staining is ≤2.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  2. Weymar GHJ, et al. 2022. Cell Rep. 40:111311. PubMed
  3. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  4. Kerkman PF, Kempers AC 2016. Ann Rheum Dis. 75(12):2201-2207. PubMed
  5. Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
  6. Yarzabek B et al. 2018. eLife. 7 pii: e34961. PubMed
  7. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  8. Geng J, et al. 2018. Elife. 7:e36341. PubMed
  9. Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
  10. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
  11. Alter G, et al. 2020. Cell. 183(1):185-196.e14. PubMed
  12. Tjwa E, et al. 2011. J Hepatol. 54:209. PubMed
  13. Ye C, et al. 2017. J Virol. 91:e01389-23. PubMed
  14. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  15. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  16. Fu W, et al. 2016. Sci Rep. 6:38162. PubMed
  17. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  18. Bego M, et al. 2015. J Vis Exp. 103: 51207. PubMed
  19. Hesketh AJ et al. 2015. PloS one. 10(12):e0145197 . PubMed
  20. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
  21. Schoutrop E, et al. 2022. Oncoimmunology. 11:2093426. PubMed
  22. Gawel DR, et al. 2019. Genome Med. 11:47. PubMed
  23. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  24. Horwitz JA, et al. 2017. Cell. 170:637. PubMed
  25. Schneider EH, et al. 2020. Naunyn Schmiedebergs Arch Pharmacol. 393:1251. PubMed
  26. Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed
  27. Rydbirk R, et al. 2019. Sci Rep. 9:7781. PubMed
  28. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  29. Wang R, et al. 2016. Proc Natl Acad Sci U S A. 113: 11501 - 11506. PubMed
  30. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  31. Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed
  32. Mock U, et al. 2015. Nucleic Acids Res. 43:5560. PubMed
  33. Richard J, et al. 2013. Virology. 443:248. PubMed
  34. Grant EJ, et al. 2018. Nat Commun. 9:5427. PubMed
  35. Artinger M, et al. 2021. Int J Mol Sci. 22: . PubMed
RRID
AB_493095 (BioLegend Cat. No. 300418) AB_1595437 (BioLegend Cat. No. 300431) AB_493094 (BioLegend Cat. No. 300417) AB_2562048 (BioLegend Cat. No. 300442)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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