Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.?

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7?and formalin-fixed paraffin-embedded sections¹¹, immunoprecipitation¹, activation of T cells^2,3,5, and Western blotting⁹. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

1. Salmeron A,?et al.?1991.?J. Immunol.?147:3047. (IP)
2. Graves J,?et al.?1991.?J. Immunol.?146:2102. (Activ)
3. Lafont V,?et al.?2000.?J. Biol. Chem.?275:19282. (Activ)
4. Ryschich E,?et al.?2003.?Tissue Antigens?62:48. (IHC)
5. Thompson AG,?et al.?2004.?J. Immunol.?173:1671. (Activ)
6. Sakkas LI,?et al.?1998.?Clin. Diagn. Lab. Immun.?5:430. (IHC)
7. Mack CL,?et al.?2004.?Pediatr. Res.?56:79. (IHC)
8. Thakral D,?et al.?2008.?J. Immunol.?180:7431. (FC)?PubMed
9. Van Dongen JJM,?et al.?1988.?Blood?71:603. (WB)
10. Yoshino N,?et al.?2000.?Exp. Anim. (Tokyo)?49:97. (FC)
11. Pollard, K.?et al. 1987.?J. Histochem. Cytochem. 35:1329. (IHC)
12. Luckashenak N,?et al.?2013.?J. Immunol.?190:27.?PubMed
13. Laurent AJ,?et al.?2014.?PLoS One.?9:103683.?PubMed
14. Li J,?et al.?2015.?Cancer Res.?75:508.?PubMed
15. Stoeckius M,?et al.?2017.?Nat. Methods.?14:865-868. (PG)

GMP RUO Flow Cytometry Antibodies.?BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

• Batch-to-batch consistency
• Material traceability
• Documented procedures
• Documented employee training
• Equipment maintenance and monitoring records
• Lot-specific certificates of analysis
• Quality audits per ISO 13485:2016
• QA review of released products

Mature T and NK T cells, thymocyte differentiation

1. Barclay N,?et al.?1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P,?et al.?1981.?Eur?J?Immunol.?11:329-34.
3. Lanier L,?et al.?1986.?J?Immunol.?137:2501-2507.