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Brilliant Violet 510™ anti-human CD3 Antibod
产品名称：
Brilliant Violet 510™ anti-human CD3 Antibod
产品类别：
抗体
产品编号：
300447
产品应用：
300447
[价格]
规格 价格 库存
25tests ￥ 2702 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Jiang XL, et al. 2021. Nat Commun. 12:897. PubMed
  2. Mukhamedova M, et al. 2021. Immunity. 54(4):769-780.e6. PubMed
  3. Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
  4. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  5. Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
  6. Wallin J, et al. 2016. Nat Commun. 7:12624. PubMed
  7. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  8. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  9. Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed
  10. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  11. Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
  12. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  13. Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed
  14. Frei A, et al. 2016. Nat Methods. 10.1038/nmeth.3742. PubMed
  15. Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed
  16. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
  17. Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
  18. K?rner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  19. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  20. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  21. Wilmes S, et al. 2021. Elife. 10:. PubMed
  22. Lemarquis AL, et al. 2019. Front Immunol. 10:403. PubMed
  23. Gorby C, et al. 2020. Sci Signal. :13. PubMed
  24. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  25. Faissner S, et al. 2022. Front Immunol. 13:980526. PubMed
RRID
AB_2563467 (BioLegend Cat. No. 300447) AB_2563468 (BioLegend Cat. No. 300448)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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