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Brilliant Violet 421™ anti-human CD3 Antibod
产品名称：
Brilliant Violet 421™ anti-human CD3 Antibod
产品类别：
抗体
产品编号：
300433
产品应用：
300433
[价格]
规格 价格 库存
25tests ￥ 2660 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L?per million cells in 100 ?L?staining volume or 5 ?L?per 100 ?L?of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Additional Product Notes

View more applications data using this product in flow cytometry and to study phosphorylation kinetics in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Lee Y, et al. 2020. J Innate Immun. 1:. PubMed
  2. Yoshitomi H, et al. 2018. Nat Commun. 2.9875. PubMed
  3. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  4. Girard MC, et al. 2021. Sci Rep. 11:5511. PubMed
  5. Mimitou EP, et al. 2021. Nat Biotechnol. 39:1246. PubMed
  6. Svensson A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183268. PubMed
  7. Ott PA, et al. 2017. Nature. 547:217. PubMed
  8. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  9. Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed
  10. Such L, et al. 2020. J Clin Invest. 4266:130. PubMed
  11. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  12. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  13. Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
  14. Saluzzo S, et al. 2022. STAR Protoc. 3:101470. PubMed
  15. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  16. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  17. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  18. Georg P, et al. 2022. Cell. 185:493. PubMed
  19. Merkt W, et al. 2021. BMC Immunol. 22:26. PubMed
  20. Lanz AL, et al. 2021. Cell Reports. 36(2):109375. PubMed
  21. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  22. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
  23. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
  24. Baguma R, et al. 2017. PLoS One. 10.1371/journal.pone.0184563. PubMed
RRID
AB_10897105 (BioLegend Cat. No. 300433) AB_10962690 (BioLegend Cat. No. 300434)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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