热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
APC/Cyanine7 anti-human CD3 Antibody
产品名称：
APC/Cyanine7 anti-human CD3 Antibody
产品类别：
抗体
产品编号：
300425
产品应用：
300425
[价格]
规格 价格 库存
25tests ￥ 2002 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  2. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  3. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  4. Woldemeskel BA, et al. 2020. J Clin Invest. 130:6631. PubMed
  5. Wang K, et al. 2020. Signal Transduct Target Ther. 0.404861111. PubMed
  6. Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
  7. Woldemeskel BA, et al. 2021. J Clin Invest. 131:. PubMed
  8. Panwar B, et al. 2021. Genome Res. 31:659. PubMed
  9. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  10. Singh NK, et al. 2022. Nat Commun. 13:7189. PubMed
  11. Ollé Hurtado M, et al. 2019. PLoS One. 14:e0216373. PubMed
  12. Buchan SL et al. 2018. Immunity. 49(5):958-970 . PubMed
  13. Das J, et al. 2021. Med (N Y). 2:1269. PubMed
  14. Xia Y, et al. 2019. Gastroenterol Res Pract. 2019:5436961. PubMed
  15. García-Espinoza JA, et al. 2022. Curr Issues Mol Biol. 44:764. PubMed
  16. Woldemeskel BA, et al. 2022. J Clin Invest. 132:. PubMed
  17. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  18. Minassian AM, et al. 2021. Med (N Y). 2:701. PubMed
  19. Lee J, et al. 2020. Sci Rep. 10:17753. PubMed
  20. Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
  21. Mu Z, et al. 2021. Genome Biol. 122:22. PubMed
  22. Sharkey A, et al. 2015. J Immunol. 195: 3026 - 3032. PubMed
  23. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  24. Sokal A, et al. 2021. Cell. 184(5):1201-1213.e14. PubMed
  25. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  26. Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed
  27. Sim MJW, et al. 2020. Proc Natl Acad Sci U S A. 117:12826. PubMed
  28. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  29. Atyeo C, et al. 2022. Nat Commun. 13:3571. PubMed
  30. Dykema AG, et al. 2021. J Clin Invest. 131:. PubMed
  31. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  32. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  33. Sokal A, et al. 2021. Immunity. S1074-7613:00396. PubMed
RRID
AB_830754 (BioLegend Cat. No. 300425) AB_830755 (BioLegend Cat. No. 300426)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线