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Alexa Fluor® 488 anti-human CD3 Antibody
产品名称：
Alexa Fluor® 488 anti-human CD3 Antibody
产品类别：
抗体
产品编号：
300454
产品应用：
300454
[价格]
规格 价格 库存
100µg ￥ 3458 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
?g size: 0.5 mg/mL
test size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume For flow cytometric staining using the test size, the suggested use of this reagent is 5 ?L?per million cells in 100 ?L?staining volume or 5 ?L?per 100 ?L?of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  17. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. ?zcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed
  2. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  3. Li C, et al. 2022. J Exp Med. 219:. PubMed
  4. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  5. Kerstein A, et al. 2016. J Autoimmun. S0896-8411(16)30186-X. PubMed
  6. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  7. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  8. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  9. VanSaun M, et al. 2013. PLoS One. 8:73054. PubMed
  10. Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed
  11. Marquardsen F, et al. 2017. J Clin Immunol. 10.1007/s10875-017-0431-5. PubMed
  12. Burel JG, et al. 2019. Elife. 8. PubMed
  13. Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed
  14. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  15. Wallace JG, et al. 2021. J Allergy Clin Immunol. 147:743. PubMed
  16. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  17. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  18. Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
RRID
AB_2564149 (BioLegend Cat. No. 300454) AB_389310 (BioLegend Cat. No. 300415)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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