Purified anti-human CD45RO Antibody
- 产品名称：
- Purified anti-human CD45RO Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304202
- 产品应用：
- 304202
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cynomolgus, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- IL-2 dependent T cell line, CA1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
WB, IP, IHC-P - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and na?ve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
- Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
- Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
- Yamada M, et al. 1996. Stroke 27:1155. (IHC)
- Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
- Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
- Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
- Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
- Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
- Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
- Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
- Delgobo M, et al. 2021. Front Immunol. 12:584538. PubMed
- Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
- Ferrian S, et al. 2021. Cell Rep Med. 2:100419. PubMed
- Guo T, et al. 2018. J Immunol. 200:500. PubMed
- Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
- Cosgrove PR, et al. 2021. Pediatr Res. . PubMed
- Wu Y, et al. 2007. Infect Immun. 75:4357. PubMed
- Xu-Monette ZY, et al. 2019. Cancer Immunol Res. 7:644. PubMed
- Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
- Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
- Gee MH, et al. 2018. Cell. 172:549. PubMed
- Mozaffarian N, et al. 2008. Rheumatology. 47:1335. PubMed
- Wu Y, et al. 2007. Infect Immun . 178:2802. PubMed
- Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
- Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
- Sergio Gonzalez‐Duque et al. 2018. Cell metabolism. 28(6):946-960 . PubMed
- White M, et al. 2015. J Immunol. 195: 1858-1867. PubMed
- Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
- NULL, et al. 2022. Cell. 185:916. PubMed
- Bender C, et al. 2020. Sci Adv. :6. PubMed
- Martin E, et al. 2020. JCI Insight. :5. PubMed
- Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
- Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
- RRID
- AB_314418 (BioLegend Cat. No. 304202)
- Structure
- Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
- Distribution
-
Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- CD22
- Cell Type
- B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RO on UniProt.org
