PE anti-human CD45RO Antibody
- 产品名称：
- PE anti-human CD45RO Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304205
- 产品应用：
- 304205
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cynomolgus, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- IL-2 dependent T cell line, CA1
- Formulation
- ?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.2 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and na?ve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
- Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
- Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
- Yamada M, et al. 1996. Stroke 27:1155. (IHC)
- Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
- Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
- Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
- Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
- Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
- Li M, et al. 2021. J Clin Invest. 131:. PubMed
- Lee YJ, et al. 2021. Front Immunol. 615369:12. PubMed
- Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
- Carisey AF, et al. 2018. Curr Biol. 28:489. PubMed
- Wang Z, et al. 2021. Cell Mol Immunol. 18:2188. PubMed
- Sutavani R, et al. 2013. J Immunol. 191:5895. PubMed
- Guo J, et al. 2022. Front Cell Dev Biol. 9:775599. PubMed
- Wu K, et al. 2021. Aging (Albany NY). 13:. PubMed
- Elston L, et al. 2020. Br J Haematol. 188:872. PubMed
- Iannetta M, et al. 2016. PLoS One. 11: 0160277. PubMed
- Narsale A, Moya R, Robertson H 2016. Data Brief. 8: 1348-51. PubMed
- Islas-Vazquez L, et al. 2020. Biology (Basel). 9:00. PubMed
- Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
- Murakami T, et al. 2018. Nat Commun. 9:2436. PubMed
- Wu D, et al. 2020. Biomark Res. 8:3. PubMed
- Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
- Godbersen C, et al. 2017. Mol Cancer Ther. 16:1335. PubMed
- Hirama T, et al. 2021. JCI Insight. 6:. PubMed
- Zhao B, et al. 2013. PLoS One. 8:77708. PubMed
- Zhou Z, et al. 2022. J Gastrointest Oncol. 13:732. PubMed
- Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
- Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
- Li M, et al. 2020. Nat Commun. 4051:11. PubMed
- Shan L, et al. 2017. Immunity. 47:766. PubMed
- Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
- RRID
- AB_314421 (BioLegend Cat. No. 304205) AB_314422 (BioLegend Cat. No. 304206) AB_2564160 (BioLegend Cat. No. 304244)
- Structure
- Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
- Distribution
-
Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- CD22
- Cell Type
- B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RO on UniProt.org
