GMP APC anti-human CD45RO Antibody
- 产品名称：
- GMP APC anti-human CD45RO Antibody
- 产品类别：
- 抗体
- 产品编号：
- 260052
- 产品应用：
- 260052
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 4914 |
1 |
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- IL-2 dependent T cell line, CA1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 100 ?g/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and na?ve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5?and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W,?et al.?Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
- Ishii T,?et al.?2001.?P. Natl. Acad. Sci. USA?98:12138. (WB)
- Ponsford M,?et al.?2001.?Clin. Exp. Immunol.?124:315. (IP)
- Yamada M,?et al.?1996.?Stroke?27:1155. (IHC)
- Sakkas LI,?et al.?1998.?Clin. Diagn. Lab. Immunol.?5:430. (IHC)
- Baba N,?et al.?2010.?Int. Immunol.?22:237.?PubMed
- Thakral D,?et al.?2008.?J. Immunol.?180:7431. (FC)?PubMed
- Weiss L,?et al.?2010.?P. Natl. Acad. Sci. USA?107:10632.?PubMed
- Wu YY,?et al.?2007.?Infect. Immun.?75:4357.?PubMed
- Mozaffarian N,?et al.?2008.?Rheumatology?47:1335.?PubMed
- Roque S,?et al.?2007.?J. Immunol.?178:8028.?PubMed
- Yoshino N,?et al.?2000.?Exp. Anim. (Tokyo)?49:97. (FC)
- Smith SH,?et al.?1986.?Immunology?58:63. (Immunogen)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- RRID
- AB_2910361 (BioLegend Cat. No. 260052)
- Disclaimer
GMP RUO Flow Cytometry Antibodies.?BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
- Structure
- Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
- Distribution
-
Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- CD22
- Cell Type
- B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Thomas M. 1989.?Annu?Rev?Immunol.?7:339-69.
- Trowbridge I,?et al.?1994.?Annu?Rev Immunol.?12:85-116.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RO on UniProt.org
