APC anti-human CD45RO Antibody
- 产品名称：
- APC anti-human CD45RO Antibody
- 产品类别：
- 抗体
- 产品编号：
- 304210
- 产品应用：
- 304210
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 2086 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cynomolgus, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- IL-2 dependent T cell line, CA1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and na?ve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
- Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
- Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
- Yamada M, et al. 1996. Stroke 27:1155. (IHC)
- Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
- Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
- Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
- Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
- Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Adams S, et al. 2012. Clin Cancer Res. 18:6748. PubMed
- Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed
- Liu F, et al. 2020. Oncoimmunology. 9:1746573. PubMed
- Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
- Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
- Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed
- Wang J, et al. 2020. Cancer Immunol Res. 794:8. PubMed
- Gullà A, et al. 2021. Cancer Discov. . PubMed
- Lee HJ, et al. 2017. Oncotarget. 8:113345. PubMed
- Zebley CC, et al. 2021. Cell Rep. 37:110079. PubMed
- Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
- Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
- Gullà A, et al. 2021. Cancer Discov. 2:468. PubMed
- Tokatlian T, et al. 2022. J Immunother Cancer. 10:. PubMed
- Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
- RRID
- AB_314426 (BioLegend Cat. No. 304210)
- Structure
- Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
- Distribution
-
Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- CD22
- Cell Type
- B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RO on UniProt.org
