Ultra-LEAF™ Purified anti-mouse TCR γ/-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, Stim, Depletion - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro stimulation^1,2,4. Although several papers have reported in vivo depletion^{3, 5,6}, another study found that the effect of the antibody could be the functional blocking of the TCR signaling, rather than cell depletion⁷. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107515 - 107520).

Though clone UC7-13D5 has been used for the depletion of TCR γ/δ T cells in vivo^3-5, there is a report suggesting that binding of clone UC7-13D5 to the γ/δ TCR leads to downregulation of the γ/δ TCR, making the γ/δ T cells undetectable although they are still present⁷.

Application References (PubMed link indicates BioLegend citation)

Bluestone JA, et al. 1991. Immunol. Rev. 120:5. (IP Costim)
Kruisbeek AM, et al. 1991. In Current Protocols in Immunology p. 3.12.1 - 3.12.14. (Costim)
Hiromatsu K, et al. 1992. J. Exp. Med. 175:49. (Deplete)
Dieli F, et al. 1997. Eur. J. Immunol. 27:206. (Costim)
van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
Pollinger B, et al. 2011. J. Immunol. 186:2602. PubMed
Koenecke C, et al. 2009. Eur J Immunol. 39(2):372-9.

Product Citations

Yang Y, et al. 2021. Front Immunol. 12:674542. PubMed

RRID

AB_2813962 (BioLegend Cat. No. 107515) AB_2813963 (BioLegend Cat. No. 107516) AB_2813964 (BioLegend Cat. No. 107517) AB_2813965 (BioLegend Cat. No. 107518) AB_2813966 (BioLegend Cat. No. 107519) AB_2813967 (BioLegend Cat. No. 107520)

Antigen Details

Structure: Ig superfamily, associates with CD3 complex
Distribution: T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
Function: Antigen recognition
Ligand/Receptor: Some bacterial or tumor antigens bound MHC class I
Cell Type: Epithelial cells, T cells, Tregs
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Skarstein K, et al. 1995. Immunology 81:497.
2. Harrison LC, et al. 1996. J. Exp. Med. 184:2167.
3. Wildner G, et al. 1996. Eur. J. Immunol. 26:2140.
4. Brandes M, et al. 2005. Science 309:264.
Gene ID: 110066 View all products for this Gene ID 110067 View all products for this Gene ID
UniProt: View information about TCR gamma/delta on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们