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PE anti-mouse CD273 (B7-DC, PD-L2) Antibody
产品名称：
PE anti-mouse CD273 (B7-DC, PD-L2) Antibody
产品类别：
抗体
产品编号：
107205
产品应用：
107205
[价格]
规格 价格 库存
50µg ￥ 1876 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse B7-DC transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1,6, blocking2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections3. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107221-107226).

Application References

(PubMed link indicates BioLegend citation)
  1. Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC, IP, WB)
  2. Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block)
  3. Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC)
  4. Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC, Block)
  5. Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block)
  6. Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed
  7. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  8. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Ochiai S, et al. 2014. J Immunol. 193:2504. PubMed
  2. Lakins MA, et al. 2018. Nat Commun. 9:948. PubMed
  3. Yonemitsu K, et al. 2022. Sci Rep. 12:12007. PubMed
  4. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
  5. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  6. Pack AD, et al. 2021. Cell Reports. 36(8):109586. PubMed
  7. Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed
  8. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  9. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  10. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  11. Jelinek D, et al. 2014. J Immunol. 192:3548. PubMed
  12. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  13. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  14. Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed
  15. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  16. Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed
  17. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  18. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  19. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_2299418 (BioLegend Cat. No. 107205) AB_2162011 (BioLegend Cat. No. 107206)

Antigen Details

Structure
B7 Immunoglobulin superfamily, 42 kD
Distribution

Dendritic cells, liver, few transformed cell lines, subset of macrophages; not expressed on lymphoid tissues

Function
Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor
PD-1, uncharacterized receptor
Cell Type
Dendritic cells, Macrophages
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Shin T, et al. 2003. J. Exp. Med. 198:31.
2. Liu X, et al. 2003. J. Exp. Med. 197:1721.
3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
58205 View all products for this Gene ID
UniProt
View information about CD273 on UniProt.org

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