Brilliant Violet 421™ anti-mouse CD273 (B7-D-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 3388	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse B7-DC transfected cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting^1,6, blocking^2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections³. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107221-107226).

Application References (PubMed link indicates BioLegend citation)

Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC, IP, WB)
Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block)
Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC)
Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC, Block)
Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block)
Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Murakami R, et al. 2013. PLoS One. 8:73270. PubMed

Product Citations

Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
Mao C, et al. 2020. Antib Ther. 0.234027778. PubMed
Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed

RRID

AB_2728127 (BioLegend Cat. No. 107219)

Antigen Details

Structure: B7 Immunoglobulin superfamily, 42 kD
Distribution: Dendritic cells, liver, few transformed cell lines, subset of macrophages; not expressed on lymphoid tissues
Function: Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor: PD-1, uncharacterized receptor
Cell Type: Dendritic cells, Macrophages
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Shin T, et al. 2003. J. Exp. Med. 198:31.
2. Liu X, et al. 2003. J. Exp. Med. 197:1721.
3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
Gene ID: 58205 View all products for this Gene ID
UniProt: View information about CD273 on UniProt.org

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