PerCP/Cyanine5.5 anti-mouse CD73 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD73 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 127213
- 产品应用：
- 127213
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CHO cells transfected with mouse CD73
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
- Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
- Product Citations
-
- Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
- Dumas SJ, et al. 2021. STAR Protocols. 2(3):100523. PubMed
- Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
- Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed
- Duan L, et al. 2021. Immunity. 54:2256. PubMed
- Mintz MA, et al. 2019. Immunity. 51:310. PubMed
- Dumas SJ, et al. 2020. J Am Soc Nephrol. 31:118. PubMed
- Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
- Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
- Raso F, et al. 2018. J Clin Invest. 128:4163. PubMed
- Young JS, et al. 2018. Front Immunol. 9:1385. PubMed
- Ott M, et al. 2020. JCI Insight. 5(17):134386. PubMed
- RRID
- AB_11219608 (BioLegend Cat. No. 127213) AB_11219403 (BioLegend Cat. No. 127214)
- Structure
- 69 kD GPI-anchored surface protein
- Distribution
-
Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
- Function
- A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
- Ligand/Receptor
- Nucleoside 5' monophosphate
- Cell Type
- B cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.
- Regulation
- Expression is regulated by the development and maturation of leukocytes
- Gene ID
- 23959 View all products for this Gene ID
- UniProt
- View information about CD73 on UniProt.org
