Pacific Blue™ anti-mouse CD73 Antibody
- 产品名称：
- Pacific Blue™ anti-mouse CD73 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 127211
- 产品应用：
- 127211
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CHO cells transfected with mouse CD73
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
- Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
- Product Citations
-
- Bergot AS, et al. 2020. J Immunol. 204:1787. PubMed
- Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed
- Garhyan J, et al. 2015. Am J Pathol. 185: 1924-1934. PubMed
- Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
- Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
- Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
- Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
- RRID
- AB_11218608 (BioLegend Cat. No. 127211) AB_11219190 (BioLegend Cat. No. 127212)
- Structure
- 69 kD GPI-anchored surface protein
- Distribution
-
Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
- Function
- A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
- Ligand/Receptor
- Nucleoside 5' monophosphate
- Cell Type
- B cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.
- Regulation
- Expression is regulated by the development and maturation of leukocytes
- Gene ID
- 23959 View all products for this Gene ID
- UniProt
- View information about CD73 on UniProt.org
