Brilliant Violet 605™ anti-mouse CD73 Antibo
- 产品名称：
- Brilliant Violet 605™ anti-mouse CD73 Antibo
- 产品类别：
- 抗体
- 产品编号：
- 127215
- 产品应用：
- 127215
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CHO cells transfected with mouse CD73
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
- Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
- Product Citations
-
- Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
- Andrade MS, et al. 2022. JCI Insight. 7:. PubMed
- Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
- Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
- Perrot I et al. 2019. Cell Rep. 27(8):2411-2425 . PubMed
- Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
- Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
- Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
- Abbott RK, et al. 2018. Immunity. 48:133. PubMed
- Quiroz HJ, et al. 2022. Front Cardiovasc Med. 8:826687. PubMed
- Watson MJ, et al. 2021. Nature. 591:645. PubMed
- Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
- Jones D, et al. 2015. J Immunol. 195: 4753 - 4759. PubMed
- Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
- RRID
- AB_2561528 (BioLegend Cat. No. 127215)
- Structure
- 69 kD GPI-anchored surface protein
- Distribution
-
Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
- Function
- A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
- Ligand/Receptor
- Nucleoside 5' monophosphate
- Cell Type
- B cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.
- Regulation
- Expression is regulated by the development and maturation of leukocytes
- Gene ID
- 23959 View all products for this Gene ID
- UniProt
- View information about CD73 on UniProt.org
