Ultra-LEAF™ Purified anti-mouse/human CD351
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse/human CD351
- 产品类别：
- 抗体
- 产品编号：
- 137307
- 产品应用：
- 137307
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- mFcα/? receptor transfected Ba/F3 cells
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature
- Recommended Usage
Each lot of this antiboday is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported (for relevant formats) application include: blocking binding of both IgM and IgA to the mFcα/? receptor2.
- Application References
(PubMed link indicates BioLegend citation) -
- Cho Y, et al. 2006. Biochem. Biophys. Res. Commun. 345:474.
- Shibuya A, et al. 2006. Springer Semin. Immunopathol. 28:377.
- Anguetil F, et al. 2015. J Immunol. 194:3664. PubMed
- RRID
- AB_2888886 (BioLegend Cat. No. 137307) AB_2888886 (BioLegend Cat. No. 137308)
- Structure
- About 70 kD transmembrane glycoprotein with an Ig-like domain in extracellular portion.
- Distribution
-
B cells, macrophages, and activated T cells.
- Function
- Mediate primary B lymphocyte endocytosis and involve in the primary stages of the immune response to microbes.
- Ligand/Receptor
- IgA and IgM
- Cell Type
- B cells, Macrophages, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
1. Shibuya A, et al. 2000. Nat. Immunol. 1(5):441.
2. Sandor M, et al. 1992. J. Immunol.. 148(8):2363.
3. Cho Y, et al. 2010. Mol. Immunol. 47(4):878.
- Gene ID
- 14125 View all products for this Gene ID 83953 View all products for this Gene ID
- UniProt
- View information about CD351 on UniProt.org
