Ultra-LEAF™ Purified anti-mouse CD300a Antib
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD300a Antib
- 产品类别：
- 抗体
- 产品编号：
- 147901
- 产品应用：
- 147901
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- MAIR-I transfected
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, Activ - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for relevant formats) include: immunoprecipitation and induction of cytokine production by peritoneal macrophages.
- Application References
(PubMed link indicates BioLegend citation) -
- Nakahashi-Oda C, et al. 2012. J. Exp. Med. 209:8. (FC, Block)
- RRID
- AB_2563668 (BioLegend Cat. No. 147901)
- Structure
- Ig superfamily, three immunoreceptor tyrosine-based inhibitory motifs (ITIMs), one non-canonical ITIM
- Distribution
-
Peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells
- Function
- Acts as an inhibitory receptor
- Interaction
- DAP12, DAP10, FcRγ
- Ligand/Receptor
- Phosphatidylserine
- Cell Type
- B cells, Dendritic cells, Eosinophils, Macrophages, Mast cells, Neutrophils
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Yotsumoto K, et al. 2003. J. Exp. Med. 198:223.
2. Martinez-Barriocanal A, et al. 2010. J. Biol. Chem. 285:41781.
3. Borrego F. 2013. Blood. 121:1951.
4. Lankry D, et al. 2013. Eur. J. Immunol. 43:2151.
- Gene ID
- 217303 View all products for this Gene ID
- UniProt
- View information about CD300a on UniProt.org
