Ultra-LEAF™ Purified anti-human/mouse Latent-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3780	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Mouse Latent TGF-β

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested

Recommended Usage

Concentrations of 1 ?g/mL?and 0.5 ?g/mL?of the capture antibody were utilized to generate the example standard curves for human latent TGF-β and mouse latent TGF-β, respectively. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture:
To measure human latent TGF-?, this antibody can be used as a capture antibody in sandwich ELISA format, and paired with the biotinylated TW4-6H10 antibody (Cat. No. 349707) as the detection antibody. Recombinant human latent TGF-? (ELISA Std.) (Cat. No. 593509) can be used as the protein standard.
To measure mouse latent TGF-?, this antibody can be used as a capture antibody in sandwich ELISA format, and paired with the biotinylated TW7-16B4 antibody (Cat. No. 141411) as the detection antibody. Recombinant mouse latent TGF-? (ELISA Std.) (Cat. No. 594509) can be used as the protein standard.

Note: For testing human latent TGF-? in serum, plasma or supernatant, LEGEND MAX? Human Latent TGF-? ELISA Kit (Cat. No. 432907) is specially developed and recommended. For testing mouse latent TGF-?, LEGEND MAX? Mouse Latent TGF-? ELISA Kit (Cat. No. 433007) is recommended.

Product Citations

Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
Jiang J, et al. 2021. Front Immunol. 12:785549. PubMed

RRID

AB_2888885 (BioLegend Cat. No. 361203) AB_2888885 (BioLegend Cat. No. 361204)

Antigen Details

Structure: Homodimer
Distribution: Latent TGF-β is secreted by numerous cells, and activated Treg cells.
Function: Latent TGF-β is the inactive form of TGF-β1 and LAP.
Ligand/Receptor: Latent TGF-β binding proteins (LTBPs)
Cell Type: Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Lu M, et al. 2002. J. Cell Sci. 115:4641.
2. Khalil N. 1999. Microbes. Infect. 1:1255.
3. Probst-Kepper M and Buer J. 2010. Biol. Direct 5:8.
4. Ali N, et al. 2008. PLoS One 3:e1914.
Gene ID: 7040 View all products for this Gene ID
UniProt: View information about Latent TGF-beta on UniProt.org

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