Ultra-LEAF™ Purified anti-mouse/human LAP (T-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2394	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Mouse Tgfb1'?transduced P3U1 cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
WB, IP, Neut - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.² TW7-28G11 recognizes conformation specific latent TGF-β complex (LAP + TGF-β) and pro-TGF-β1, but not free LAP or active TGF-β.

Additional reported applications (for relevant formats) include: Western blotting¹, immunoprecipitation¹, and neutralization².?TW7-28G11 is able to neutralize TGF-β activity.

Application References (PubMed link indicates BioLegend citation)

Oida T and Weiner HL. 2010. PloS ONE 5:e15523. (FC, IP, WB)
Oida T and Weiner HL. 2011. PLoS ONE 6:e18365. (Neut)

Product Citations

Dong X, et al. 2017. Nature. 542:55-59. PubMed

RRID

AB_2562709 (BioLegend Cat. No. 146703) AB_2562710 (BioLegend Cat. No. 146704)

Antigen Details

Structure: Dimers of latency-associated peptide non-covalently associated with dimers of mature TGF-β
Distribution: Many cell types, highly expressed on activated Treg cells and platelets
Function: TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response.
Ligand/Receptor: TGF-β receptors
Cell Type: Tregs, Platelets, Dendritic cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Oida T and Weiner HL.?2010. PLoS One 5:e15523.
2. Tran DQ, et al. 2009. Proc. Natl. Acad. Sci. USA 106:13445.?
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.?
5. Nakamura K, 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factor 8:11.
Gene ID: 21803 View all products for this Gene ID
UniProt: View information about LAP on UniProt.org

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