Ultra-LEAF™ Purified anti-mouse LAP (TGF-&be-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3780	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Mouse Tgfb1-transduced P3U1 cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, Neut, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone TW7-20B9 has been reported to not cross-react with human LAP.² Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.² This antibody recognizes recombinant LAP, latent TGF-?, and pro-TGF-?.

Additional reported applications (for relevant formats) include: Western blotting¹, immunoprecipitation¹, and neutralization². TW7-20B9 is able to neutralize certain unconventional T cell-derived forms of TGF-? activity, but not the distinct 25 kDa free form of TGF-?.²

Application References (PubMed link indicates BioLegend citation)

Oida T, et al. 2010. PLoS ONE (FC, IP, WB)
Oida T, et al. 2011. PLoS ONE 6:e18365. (Neut)
Tu Z, et al. 2012. Invest Ophthalmol Vis Sci. 53:959. PubMed

RRID

AB_2832509 (BioLegend Cat. No. 141307) AB_2860693 (BioLegend Cat. No. 141308)

Antigen Details

Structure: Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Distribution: Many cell types, highly expressed on activated Tregs and platelets
Function: TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response.
Ligand/Receptor: TGF-β receptors
Cell Type: Dendritic cells, Platelets, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Oida T, et al. 2010. PLoS
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.
Gene ID: 21803 View all products for this Gene ID
UniProt: View information about LAP on UniProt.org

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